Recombinant Human IL-7 GMP Protein, CF

Catalog #: BT-007-GMP

Product Specifications

Purity:>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining. The molecular weight by mass spectrometry is 17507 Da ± 50 DaEndotoxin Level：<0.10 EU per 1 μg of the protein by the LAL method.

Activity：Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.100-0.500 ng/mL.The specific activity of Recombinant Human IL-7 is >1.00 x 108 units/mg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530).

Source：E. coli-derived human IL-7 protein

Asp26-His177, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Accession #：P13232.1

N-terminal Sequence Analysis: Met-Asp26-(Cys)-Asp-Ile-Glu-Gly-Lys-Asp-Gly

Predicted Molecular Mass:17 kDa

SDS-PAGE: 17 kDa, under reducing conditions